Research & Development/Project
 

Cell Respiration Assay System (CRAS)

 
CRAS Overview
 
Measurement
~Accurate measurement by using electrochemical measurement technology
 
Non-Invasive
~Non-invasive measurement of cell's oxygen consumption (respiratory volume)
 
Objective Assessment
~More objective quality assessment than morphological observation
 

The Cell Respiration Assay System (CRAS) is a fine assay system that utilizes an electrochemical measurement technology.
This system consists of a potentiostat, inverted microscope, and internal respiratory measurement PC software.
By scanning a microprobe, it electrochemically measures the concentration gradient of dissolved oxygen in the offshore and proximity of the sample and calculates its respiratory volume using a spherical diffusion formula. The plate used in this measurement has six inverted conical wells, which allow consecutive measurements.
This device measures the oxygen consumption non-invasively by scanning the microprobe through the proximity of the sample. Its measurement method does not affect the cell's respiratory activity or embryogenesis.

 
Quality Assessment through Measurement
 
 
The diagram above shows the measurement method. The respiratory activity of one cell (one fertilized egg) can be measured by locating the microprobe by the sample placed at the center of the well and running the microprobe up and down.
※Click on the image to enlarge
The respiratory volume through the mouse embryogenesis process is shown to be increased along with the progress of the development.
 
※Click on the image to enlarge
※Click on the image to enlarge
The diagrams above show the respiratory volume of mouse blastocyst grown in different culture solutions and a comparison of their cell count.
While there is no difference in the cell count of the blastocysts between the medium cultures of Company A and Company B, a difference in their respiratory volume is observed.
The culture solution of Company C showed a result of low cell count and respiratory volume.
Combined with the use of morphological observation devices, CRAS can more accurately assess the cell's embryonic activities that cannot be observed through other existing devices. This device is also used in the embryonic transplantation of livestock animals.
 
●Reference
Abe H and Hoshi H, J Reprod Dev. 2003 Jun;49(3):193-202
Abe H, J Mamm Ova Res. 2007 Dec;24(3):70-78
Shiku H et al.,Anal Chem. 2001 Aug 1;73(15):3751-8